Sudha A , P. Latha.
Page No : 1-4
Growth and yield Response of Soybean (Glycine Max L.) in Relation to Temperature, Photoperiod and Sunshine Duration
Field experiment was conducted during kharif season of 2008 and 2009 to study the heat units requirement during different phenophases of soybean (Glycine Maax L.). Three varieties of soybean (JS 335, JS 93-05and 97-52) were sown under different environment created through different sowing time (first and third week of July and first week of August) The growing degree days (GDD). heliothemal units (HTU), photo thermal unit(PTU) and heat use efficiency (HUE) were work out for different phenophase. Analysis showed the early sown crop (first week of July) accumulated higher GDD, HTU, PTU and result in maximum HUE. Among the varieties highest value of heat units and HUE was found in cv. JS 97-52.
Priyanka Joyjit , Souradip Das.
Page No : 5-7
Influence of acid acclimation on the Alanine aminotransferase activity in fresh water mussel, Lamellidens marginalis (L).
The enzymes are the proteins with immense capabilities of metabolic turnover. The hydrogen ion concentration (pH) of any media is the key factor to orchestrate the progression of velocity of enzyme catalyzed biochemical reaction. The fresh water mussel, Lamellidens marginalis (L) was subjected to sublethal acidic (pH=4.0). Water for 16 days & its impact on the gill kinetic parameters of Alanine Amino transferase (ALAT) was analyzed. The gill ALAT activity exhibited increased pattern at all the substrate concentration in the acclimated condition over that of control. The acclimated lamelliden gill exhibited higher Vmax & lesser Km over the control. The pH dependency studied on the gill tissue ALAT enzyme showed 7.6 optimum pH for control & 7.2 for experimental groups. The acidic shift of optimum pH for enzyme after acclimation of was the significant. The thermal dependency of the enzyme activity exhibited higher activity levels of the enzyme in experimental tissue at all the temperature over the control. It is concluded that the acclimation of fresh water mussel, Lamellidens marginalis (L) to acidic environment seems to be inducing at the molecular level leading to altered metabolic pattern which provided adaptive mechanism for the positive survival even under adverse conditions.
Umamaheswari Devi .
Page No : 8-11
Production of polyclonal antibody for the detection of Cardamom mosaic virus affecting cardamom (Elettaria cardamomum Maton)
Katte disease of cardamom is one of the most destructive viral diseases caused by Cardamom mosaic virus (CdMV) affecting cardamom plantations worldwide. The disease spreads through the use of infected rhizomes or suckers as planting material. The present study was undertaken to develop polyclonal antibody against CdMV for easy and early detection of the virus in planting material. The katte infected samples were collected from Wayanad and Idukki district of Kerala and maintained under insect proof net. The virus was purified from the infected leaf using potassium phosphate buffer and analyzed in SDS-PAGE where, protein band of size 37 kDa was observed in the infected samples. Such bands were absent in healthy samples. The purified protein was then used as antigen for production of polyclonal antibodies in 6-9 month old rabbit. The antibody titre was determined using ODD (Ouchterlony Double Diffusion) assay. The antibody produces had titre of 1:10, 1:100 and 1:150 with ability to form insoluble antigen antibody complex in ODD assay. These antibody titres were then used in indirect ELISA (Enzyme linked Immuno Assay Sorbent) for detecting the CdMV in the infected leaves of cardamom plants. The result of ELISA revealed that the virus can be easily detected in 1:100 dilution of the antibody. The antibody produced can be used for indexing of the plant material for supply of disease free planting material and for quarantine management which will help to prevent the spread of CdMV virus.